CD3 antibody (FITC)

Catalog No. ABIN119605

This Mouse Monoclonal antibody specifically detects CD3 in FACS. It exhibits reactivity toward Rhesus Monkey.

Quick Overview for CD3 antibody (FITC) (ABIN119605)

Target: CD3

Reactivity: Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: FN-18

Product Details anti-CD3 Antibody (FITC)

Purification: Affinity Chromatography on Protein A

Immunogen: Rhesus monkey peripheral blood leucocytes. Spleen cells from immunised mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/10, Use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09 % Sodium Azide and 1 % BSA is recommended.

Buffer: PBS containing 1 % BSA and 125 mM Trehalose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 9541

UniProt: Q95LI5

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway